Journal: Cell Reports Medicine

Article Title: PARP-1 improves leukemia outcomes by inducing parthanatos during chemotherapy

doi: 10.1016/j.xcrm.2023.101191

Figure Lengend Snippet:

Article Snippet: Human: HS-5 stromal cells , LGC Standards , ATCC Cat# CRL-11882, RRID: CVCL_3720.

Techniques: Recombinant, Derivative Assay, Staining, Negative Control, Plasmid Preparation, Caspase-3 Assay, TUNEL Assay, Reverse Transcription, Expressing, Bicinchoninic Acid Protein Assay, Western Blot, Microarray, Software, Fluorescence

Journal: Communications Medicine

Article Title: Preclinical characterization of an active immunotherapy targeting calcitonin gene-related peptide

doi: 10.1038/s43856-025-00870-2

Figure Lengend Snippet: Proteome and peptide screens were used to evaluate potential off-target binding of affinity-purified p4796kb antibodies. a Manhattan plot displays results from the HuProt Human Proteome Microarray screen of antibody binding. Colors represent each block from the array, containing approximately 1000 proteins each. Spots represent individual protein intensity score from array. Hits are annotated with protein names. b Manhattan plot from peptide counter screen of 21 putative hits from proteome screen. Overlapping 15mer peptides were synthesized and printed on arrays. Spots represent individual peptide intensity scores from array. Peptide sequences of hits are displayed. Sera from guinea pigs immunized with p4796kb were tested for binding to putative hits from screens ( c ) and calcitonin family ( d ) measured by ELISA. Pre-immune sera were used as controls. Data are presented as means +/− SEM; n = 3. *** p < 0.0001.

Article Snippet: Binding of p4796kb-derived sera to 3 hits from the HuProt™ microarray screen (HSP90 beta protein, StressMarq Bioscience, SPR-102B; AKR1B10 protein, MyBioSource, MBS203315; PTPRD protein, Acro, PTD-H52H9) and to other propeptides that belong to the calcitonin/CGRP peptide family, including recombinant adrenomedullin (ADM, MyBioSource, MBS2012013), recombinant adrenomedullin 2 (ADM2, MyBioSource, MBS2123890), synthetic amylin (Abcam, ab142398), and recombinant calcitonin (Abcam, ab153793), were further evaluated.

Techniques: Binding Assay, Affinity Purification, Microarray, Blocking Assay, Synthesized, Enzyme-linked Immunosorbent Assay

Journal: Annals of Translational Medicine

Article Title: KCNQ1OT1 lncRNA affects the proliferation, apoptosis, and chemoresistance of small cell lung cancer cells via the JAK2/STAT3 axis

doi: 10.21037/atm-21-1761

Figure Lengend Snippet: KCNQ1OT1 was correlated with SCLC chemoresistance. (A) Microarray analysis of the lncRNA expression profiles showed the relative levels of lncRNA expression between sensitive (H69) and multidrug-resistant cells (H69AR). Red represents upregulated lncRNAs and blue represents downregulated lncRNAs (n=3 per group). (B) KCNQ1OT1 expression in SCLC tumor and normal tissues from the GSE43346 dataset (normal tissue samples, n=42; SCLC samples, n=23). (C) KCNQ1OT1 levels in sensitive and multidrug-resistant cells were detected by qPCR; (D) KCNQ1OT1 knockdown was validated by qPCR. (E,F) IC50 values of shKCNQ1OT1-transfected cells were measured with CCK-8 assays after exposure to ADM, DDP, and VP-16. (G,H) Cell apoptosis was determined by flow cytometric analysis. The results are presented as the mean ± SD. *, P<0.05; **, P<0.01; ***, P<0.001, compared with the control group.

Article Snippet: RNA extraction and microarray hybridization were performed by Kangchen Company (Shanghai, China) using a human lncRNA microarray (v.3.0; Arraystar, Inc., MD, USA).

Techniques: Microarray, Expressing, Knockdown, Transfection, CCK-8 Assay, Control

Journal: Journal of the American Society of Nephrology : JASN

Article Title: Progressive Renal Disease Established by Renin-Coding Adeno-Associated Virus–Driven Hypertension in Diverse Diabetic Models

doi: 10.1681/ASN.2017040385

Figure Lengend Snippet: JAK/STAT pathway is upregulated in the ReninAAV db/db uNx model of DKD. (A) Microarray analysis identified the JAK/STAT pathway as a top pathway upregulated in ReninAAV db/db uNx mice at both 8 and 12 weeks of ReninAAV treatment compared with LacZ db/db uNx controls, including Stat3, Jak2, IL6Ra, and Socs3. (B) Taqman analysis verified microarray data and showed that genes of the JAK/STAT pathway are upregulated in ReninAAV db/db uNx mice compared with LacZ controls. (C and D) Serum IL-6 and MCP-1 were upregulated in ReninAAV db/db uNx mice compared with db/m or db/db uNx controls. (E) Protein expression of pSTAT3 is upregulated in ReninAAV db/db uNx mice compared with LacZ controls. (F) Immunostaining-identified pSTAT3 is increased in renal tubule epithelium and infiltrating leukocytes compared with LacZ controls, with circles highlighting low-level detection in LacZ groups. n=5+ per group. *P<0.05 versus LacZ db/db uNx control; $P<0.05 versus db/m.

Article Snippet: Probes used were SOCS3 MM00545913_s1, IL-6 MM00446191_m1, IL6Ra MM00439653_m1, JAK1 MM00600614_m1, JAK2 MM012–8489_m1, STAT3 MM01219775_m1, and NFKB1a MM00477800_g1.

Techniques: Microarray, Expressing, Immunostaining, Control

Journal: Journal of the American Society of Nephrology : JASN

Article Title: Progressive Renal Disease Established by Renin-Coding Adeno-Associated Virus–Driven Hypertension in Diverse Diabetic Models

doi: 10.1681/ASN.2017040385

Figure Lengend Snippet: Ingenuity Pathway Analysis of ReninAAV db/db uNx and eNOS −/− db/db models

Article Snippet: Probes used were SOCS3 MM00545913_s1, IL-6 MM00446191_m1, IL6Ra MM00439653_m1, JAK1 MM00600614_m1, JAK2 MM012–8489_m1, STAT3 MM01219775_m1, and NFKB1a MM00477800_g1.

Techniques: Activation Assay, Immunopeptidomics, Protein-Protein interactions, Inhibition

Journal: Stem cell research

Article Title: Generation and characterization of human induced pluripotent stem cells (iPSCs) from three male and three female patients with CDKL5 Deficiency Disorder (CDD)

doi: 10.1016/j.scr.2021.102276

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Article Snippet: Pluripotency Marker , Rabbit anti-OCT4 , 1:100 , Abcam Cat# ab19857, RRID: AB_445175.

Techniques: Derivative Assay, Sequencing, Modification

Journal: Stem cell research

Article Title: Generation and characterization of human induced pluripotent stem cells (iPSCs) from three male and three female patients with CDKL5 Deficiency Disorder (CDD)

doi: 10.1016/j.scr.2021.102276

Figure Lengend Snippet: Characterization and validation

Article Snippet: Pluripotency Marker , Rabbit anti-OCT4 , 1:100 , Abcam Cat# ab19857, RRID: AB_445175.

Techniques: Immunocytochemistry, Staining, Expressing, Microarray, Marker, Mutagenesis, Sequencing, Southern Blot

Journal: Stem cell research

Article Title: Generation and characterization of human induced pluripotent stem cells (iPSCs) from three male and three female patients with CDKL5 Deficiency Disorder (CDD)

doi: 10.1016/j.scr.2021.102276

Figure Lengend Snippet: Reagents details

Article Snippet: Pluripotency Marker , Rabbit anti-OCT4 , 1:100 , Abcam Cat# ab19857, RRID: AB_445175.

Techniques: Immunocytochemistry, Marker, Expressing, Mutagenesis, Sequencing

Journal: Cell Stem Cell

Article Title: Regional identity of human neural stem cells determines oncogenic responses to histone H3.3 mutants

doi: 10.1016/j.stem.2021.01.016

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Article Snippet: Human GAPDH Taqman gene expression assay , ThermoFisher , Cat. Hs02758991_g1.

Techniques: Virus, Recombinant, Reverse Transcription, Protease Inhibitor, Staining, Imaging, Bicinchoninic Acid Protein Assay, Purification, Gene Expression, Protein-Protein interactions, Microarray, Expressing, CRISPR, Plasmid Preparation, Software, Magnetic Beads, Fluorsave